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. 2017 Feb 21;11:432–441. doi: 10.1016/j.dib.2017.02.046

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© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6. — E– compared with E+ embryos have impaired behavior when injected with saline (upper panel), but restored responses when injected with VitE (lower panel). A. Embryos were analyzed in 96-well plates (128 embryos per group). Locomotor activities following a series of light stimuli (a stimulus every 6 for 24 min) were measured as distance moved (mm) over time (seconds). At 96 hpf, E- (red) embryos treated with saline (upper panel) were 47% less responsive to light than were E+ embryos (E– area-under-curve, AUC: 2040±178; E+ AUC: 3877±228; p<0.0001). Embryos with morphological defects were not included in data analysis. E- behavior was restored using VitE injection into the yolk at the 1 cell stage (lower panel E– AUC: 2970±280; E+ AUC: 3340±226, not significantly different). B. Bar chart comparisons of respective time-course data. VitE (tocopherol)-injected E– and E+ embryo locomotor activities were not significantly different.