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. 2017 Feb 21;11:432–441. doi: 10.1016/j.dib.2017.02.046
Subject area Biology
More specific subject area Nutrition; Antioxidants
Type of data Graphs; Figures
How data was acquired LC-MS (liquid chromatography-mass spectrometry) using a Shimadzu Nexera system (Shimadzu; Columbia, MD, USA) coupled to a high-resolution hybrid quadrupole–time–of-flight mass spectrometer (TripleTOF® 5600; SCIEX; Framingham, MA, USA); Embryos were assessed for viability, developmental progression and spontaneous movements (earliest behavior in zebrafish), using the zebrafish acquisition and analysis program (ZAAP). Locomotor Response Assay using a Viewpoint ZebraBox (software version 3.0, Viewpoint Life Sciences, Lyon, France).
Data format Analyzed
Experimental factors Adult 5D zebrafish fed defined diets without (E–) or with VitE (E+, 500 mg RRR-α-tocopheryl acetate/kg diet) were spawned to obtain E– and E+ embryos that were evaluated up to 120 hours-post-fertilization (hpf)
Experimental features Lipid and metabolomics analyses were performed in developing E– and E+ zebrafish embryos collected daily from 24 to 120 hpf. Mortality and neurobehavioral outcomes were assessed at 96 hpf in two separate rescue experiments using E– and E+ embryo populations micro-injected into the yolk (1) at 0 hpf with VitE or (2) at 24 hpf with D-glucose
Data source location Oregon State University, Corvallis, OR 97330
Data accessibility Data is within this article