Table 1. Summary of miscalls observed in multiple software and at least twice in one software.
|
Experimental typing |
Predicted data |
Optitype |
Polysolver |
PHLAT |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene | 2nd field | 1st field | 2nd field | 1st field | No of miscalls | Error rate | P | No of miscalls | Error rate | P | No of miscalls | Error rate | P |
| HLA-A | |||||||||||||
| A*02:01 | A*02 | A*02:06 | A*02 | 3 | 0.8% | 0.25 | 1 | 0.3% | 1.0 | 4 | 1.1% | 0.12 | |
| A*02:01 | A*02 | A*02:07 | A*02 | 10 | 2.7% | 0.0018 | 9 | 2.4% | 0.0037 | 4 | 1.1% | 0.12 | |
| A*02:06 | A*02 | A*02:01 | A*02 | 7 | 18.4% | 0.012 | 5 | 13.2% | 0.054 | 4 | 10.5% | 0.12 | |
| A*02:01 | A*02 | A*26:01 | A*26 | 0 | 0% | − | 1 | 0.3% | 1.0 | 3 | 0.8% | 0.25 | |
| A*02:01 | A*02 | A*69:01 | A*69 | 1 | 0.3% | 1.0 | 2 | 0.5% | 0.50 | 0 | 0% | − | |
| A*03:01 | A*03 | A*01:01 | A*01 | 0 | 0% | − | 1 | 0.5% | 1.0 | 3 | 1.6% | 0.25 | |
| A*11:01 | A*11 | A*11:02 | A*11 | 1 | 0.6% | 1.0 | 1 | 0.6% | 1.0 | 2 | 1.2% | 0.50 | |
| A*24:04 | A*24 | A*24:02 | A*24 | 0 | 0% | − | 2 | 100.0% | 0.33 | 1 | 50.0% | 1.0 | |
| A*25:01 | A*25 | A*26:01 | A*26 | 0 | 0% | − | 14 | 93.3% | 2.1E-07 | 1 | 6.7% | 1.0 | |
| A*26:01 | A*26 | A*25:01 | A*25 | 2 | 4.1% | 0.49 | 0 | 0% | − | 1 | 2.0% | 1.0 | |
| A*26:01 | A*26 | A*26:02 | A*26 | 1 | 2.0% | 1.0 | 2 | 4.1% | 0.49 | 1 | 2.0% | 1.0 | |
| A*26:08 | A*26 | A*26:01 | A*26 | 0 | 0% | − | 2 | 66.7% | 0.40 | 3 | 100.0% | 0.10 | |
| A*29:02 | A*29 | A*29:01 | A*29 | 3 | 5.7% | 0.24 | 1 | 1.9% | 1.0 | 0 | 0% | − | |
| A*33:03 | A*33 | A*31:01 | A*31 | 1 | 1.6% | 1.0 | 0 | 0% | − | 16 | 25.4% | 1.0E-05 | |
| A*68:01 | A*68 | A*69:01 | A*69 | 0 | 0% | − | 1 | 1.9% | 1.0 | 2 | 3.8% | 0.50 | |
| HLA-B | |||||||||||||
| B*13:01 | B*13 | B*13:02 | B*13 | 1 | 4.3% | 1.0 | 1 | 4.3% | 1.0 | 8 | 34.8% | 0.0038 | |
| B*13:02 | B*13 | B*13:01 | B*13 | 6 | 12.8% | 0.026 | 2 | 4.3% | 0.49 | 0 | 0% | − | |
| B*14:01 | B*14 | B*14:02 | B*14 | 2 | 22.2% | 0.47 | 1 | 11.1% | 1.0 | 4 | 44.4% | 0.082 | |
| B*15:01 | B*15 | B*15:07 | B*15 | 0 | 0% | − | 1 | 1.4% | 1.0 | 3 | 4.1% | 0.24 | |
| B*15:15 | B*15 | B*15:01 | B*15 | 0 | 0% | − | 1 | 33.3% | 1.0 | 2 | 66.7% | 0.40 | |
| B*15:01 | B*15 | B*46:01 | B*46 | 0 | 0% | − | 2 | 2.7% | 0.50 | 2 | 2.7% | 0.50 | |
| B*27:05 | B*27 | B*08:01 | B*08 | 0 | 0% | − | 1 | 2.9% | 1.0 | 2 | 5.9% | 0.49 | |
| B*27:03 | B*27 | B*27:05 | B*27 | 3 | 75.0% | 0.14 | 1 | 25.0% | 1.0 | 1 | 75.0% | 0.14 | |
| B*35:03 | B*35 | B*35:01 | B*35 | 0 | 0% | − | 1 | 4.5% | 1.0 | 2 | 9.1% | 0.49 | |
| B*35:11 | B*35 | B*35:01 | B*35 | 0 | 0% | − | 2 | 100.0% | 0.33 | 2 | 100.0% | 0.33 | |
| B*35:12 | B*35 | B*35:02 | B*35 | 0 | 0% | − | 4 | 80.0% | 0.048 | 1 | 20.0% | 1.0 | |
| B*35:17 | B*35 | B*35:01 | B*35 | 1 | 14.3% | 1.0 | 6 | 85.7% | 0.0047 | 2 | 28.6% | 0.46 | |
| B*35:43 | B*35 | B*35:14 | B*35 | 2 | 20.0% | 0.47 | 0 | 0% | − | 2 | 20.0% | 0.47 | |
| B*35:43 | B*35 | B*49:01 | B*49 | 0 | 0% | − | 1 | 10.0% | 1.0 | 2 | 20.0% | 0.47 | |
| B*39:06 | B*39 | B*39:01 | B*39 | 2 | 12.5% | 0.48 | 1 | 6.3% | 1.0 | 11 | 68.8% | 6.8E-05 | |
| B*40:01 | B*40 | B*07:02 | B*07 | 0 | 0% | − | 1 | 0.9% | 1.0 | 12 | 10.3% | 0.00036 | |
| B*40:04 | B*40 | B*40:02 | B*40 | 1 | 25.0% | 1.0 | 3 | 75.0% | 0.14 | 3 | 75.0% | 0.14 | |
| B*40:06 | B*40 | B*40:02 | B*40 | 5 | 31.3% | 0.043 | 4 | 25.0% | 0.10 | 4 | 25.0% | 0.10 | |
| B*42:02 | B*42 | B*42:01 | B*42 | 1 | 33.3% | 1.0 | 2 | 66.7% | 0.40 | 0 | 0% | − | |
| B*44:03 | B*44 | B*44:02 | B*44 | 1 | 1.2% | 1.0 | 1 | 1.2% | 1.0 | 2 | 2.4% | 0.50 | |
| B*52:01 | B*52 | B*51:01 | B*51 | 1 | 2.1% | 1.0 | 2 | 4.2% | 0.49 | 2 | 4.2% | 0.49 | |
| B*58:01 | B*58 | B*35:01 | B*35 | 1 | 1.8% | 1.0 | 2 | 3.5% | 0.50 | 3 | 5.3% | 0.24 | |
| B*58:01 | B*58 | B*53:01 | B*53 | 1 | 1.8% | 1.0 | 3 | 5.3% | 0.24 | 1 | 1.8% | 1.0 | |
| B*58:02 | B*58 | B*53:01 | B*53 | 1 | 5.3% | 1.0 | 2 | 10.5% | 0.49 | 1 | 5.3% | 1.0 | |
| B*58:02 | B*58 | B*58:01 | B*58 | 1 | 5.3% | 1.0 | 0 | 0% | − | 10 | 52.6% | 0.00039 | |
| HLA-C | |||||||||||||
| C*02:02 | C*02 | C*02:10 | C*02 | 5 | 11.6% | 0.055 | 5 | 11.6% | 0.055 | 5 | 11.6% | 0.055 | |
| C*03:03 | C*03 | C*03:04 | C*03 | 5 | 4.9% | 0.059 | 1 | 1.0% | 1.0 | 1 | 1.0% | 1.0 | |
| C*03:04 | C*03 | C*03:02 | C*03 | 1 | 0.6% | 1.0 | 0 | 0% | − | 3 | 5.9% | 0.029 | |
| C*03:04 | C*03 | C*03:03 | C*03 | 6 | 3.7% | 0.030 | 6 | 3.7% | 0.030 | 13 | 3.9% | 0.12 | |
| C*03:03 | C*03 | C*04:01 | C*04 | 0 | 0% | − | 1 | 1.0% | 1.0 | 4 | 1.0% | 1.0 | |
| C*03:04 | C*03 | C*04:01 | C*04 | 1 | 0.6% | 1.0 | 2 | 1.2% | 0.50 | 1 | 1.8% | 0.25 | |
| C*07:01 | C*07 | C*07:02 | C*07 | 0 | 0% | − | 1 | 0.7% | 1.0 | 7 | 4.8% | 0.015 | |
| C*08:02 | C*08 | C*05:01 | C*05 | 2 | 4.1% | 0.49 | 2 | 4.1% | 0.49 | 5 | 6.1% | 0.24 | |
| C*08:01 | C*08 | C*08:03 | C*08 | 2 | 4.1% | 0.49 | 1 | 2.0% | 1.0 | 3 | 2.0% | 1.0 | |
| C*12:03 | C*12 | C*12:02 | C*12 | 1 | 1.6% | 1.0 | 4 | 6.3% | 0.12 | 4 | 6.3% | 0.12 | |
| C*14:03 | C*14 | C*14:02 | C*14 | 2 | 13.3% | 0.48 | 3 | 20.0% | 0.22 | 3 | 20.0% | 0.22 | |