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. Author manuscript; available in PMC: 2017 Dec 2.
Published in final edited form as: J Proteome Res. 2016 Oct 10;15(12):4265–4276. doi: 10.1021/acs.jproteome.6b00342

Figure 4. Western blot verifies relative protein quantitation found in SILAC proteomic results.

Figure 4

MCM5, Rb1, and p-Rb1 were down regulated while UQCRC1 was up regulated in 3D cell culture when compared to 2D. COX IV was used as a loading control throughout the experiment. Protein amounts of COX IV was confirmed to be constant using Image J.