Table 1.

Lists of AFPs used in cryopreservation of biological samples.

AFPs	Origin Species of AFPs	Cryopreserved Biological Samples		AFP Quantities Used	Freezing Methods	References
		Organisms	Sample Types
III	Fish	Turbot (Scophthalmus maximus)	Embryos	20 nL (final conc. 0.77 mg/mL) of 10 mg/mL type III AFP injected in yolk sac 0.23914 mm3)	Vitrification	[166]
I/III	Fish	Gilthead seabream (Sparus aurata)	Sperm	1 μg/mL	Vitrification	[167]
I/III/AFGP	Fish	Bull	Sperm	0.1, 1, 10, and 100 μg/mL	Cryopreservation	[168]
LeIBP	Yeast	Boar	Sperm	0.01, 0.1, and 1 mg/mL	Cryopreservation	[169]
III	Fish	Mouse	Ovarian tissue	0.1, 1, and 10 mg/mL	Vitrification	[65]
FfIBP	Bacteria
LeIBP	Yeast
AFGP	Fish	Buffalo	Sperm	0.1, 1, and 10 μg/mL	Vitrification	[170]
III	Fish	Buffalo	Sperm	0.01, 0.1, 1, and 10 mg/mL	Cryopreservation	[171]
III	Fish	Bovine	Embryos	10 mg/mL	Hypothermic	[172]
AFGP	Fish	Pig	Oocyte	40 mg/mL	Hypothermic	[83]
I	Winter flounder	Bovine	Oocyte	20 mg/mL	Hypothermic	[70]
II	Sea raven
III	Eel pout
III	Notched-fin eelpout	Human	HepG2	2–10 mg/mL	Hypothermic	[173]
I/III/AFGP	Fish	Rat	RIN-5F cells (insulinoma)	10 mg/mL	Hypothermic	[174]
I	Winter flounder	Sheep	Embryo	1 or 10 mg/mL	Hypothermic	[175]
III	Ocean pout
III	Fish	Rabbit	Sperm	0.1, 1, 10, and 100 μg/mL	Vitrification	[176]
			Embryo	100, 500, and 1000 μg/mL
III	Fish	Mouse	Oocyte	500 ng/mL	Vitrification	[177]
LeIBP	Yeast	Diatom	Diatom	0.1 mg/mL	Cryopreservation	[143]
AFGP	Fish	Carp	Spermatozoa	2–10 mg/mL	Hypothermic	[178]
I	Fish	Mouse	Pronuclear embryos, 4-cell embryos	0.1 and 1.0 mg/mL	Vitrification	[92]
III				0.1 mg/mL
I/III	Fish	Sea bream	Spermatozoa	0.1, 1, and 10 μg/mL	Cryopreservation	[165]
AFGP	Fish	Equine	Embryos	20 mg/mL	Hypothermic/Cryopreservation	[91]
DcAFP	Insect	Mouse	A10 smooth muscle cell	1 μg/mL	Cryopreservation	[179]
AFGP	Gadus morhua	Mouse	Embryos	20 mg/mL	Vitrification	[180]
III	Fish	Mouse	Ovarian tissue	0, 5, and 20 mg/mL	Vitrification	[67]
III	Fish	Mouse	Mature oocyte	2.5 mg/mL	Vitrification	[181]
I	Fish	Rat	Hippocampal slice cultures	10 mg/mL	Hypothermic	[182]
AFGP	Fish	Pig	Oocyte	40 mg/mL	Vitrification	[183]
AFGP	Fish	Mouse	Embryos	20 mg/mL	Vitrification	[183]
LeIBP	Yeast	Human	Red blood cells	0.4–0.8 mg/mL	Cryopreservation	[144]
III	Fish	Rat	Heart	3, 5, and 15 mg/mL	Hypothermic	[184]
AFGP	Fish	Rat	Cardiomyoctes	0.5–10 mg/mL	Hypothermic (−4 °C)	[162]
III	Fish	Mouse	Oocytes	500 ng/mL	Cryopreservation	[185]
AFGP	Fish	Rat	Cardiac	10 μg/mL, 10 and 15 mg/mL	Hypothermic	[164]
I/III	Fish	Zebra fish	Embryo	40 μg/mL	Hypothermic	[186]
NgIBP	Diatom	Human	Red blood cells	25, 50, and 77 μg/mL	Cryopreservation	[18]
I/III	Fish	Zebra fish	Embryo	40 μg/mL	Vitrification/Cryopreservation	[187]
DcAFP	Insect	Centipede	Gut cells	0.02 mg/mL	Cryopreservation	[188]
III	Fish	Rat	Hearts	15 mg/mL	Hypothermic	[189]
AFGP	Fish	Mouse	Oocytes	1 mg/mL	Cryopreservation	[190]
I/III	Fish	Mouse	Blastocysts	0.1, 1.0 mg/mL	Cryopreservation	[191]
I/III/AFGP	Fish	Mouse	Spermatozoa	1–100 μg/mL	Cryopreservation	[192]
AFGP	Synthetic	Rat	Islet cell	500 μg/mL	Cryopreservation	[193]
I/II/III/AFGP	Fish	Mouse	Oocytes	20 mg/mL	Vitrification	[194]
I	Fish	Human	Myelogenous leukemia cells	0~1000 μg/mL	Cryopreservation	[163]
III	Ocean pout	Chimpanzee (Pan troglodytes)	Spermatozoa	1, 10, and 100 μg/mL	Cryopreservation	[195]
I	Fish	Seabream	Embryos	20 nL of 10 mg/mL type I AFP injected	Vitrification	[196]
III	Fish	Turbot	Embryos	10 mg/mL	Hypothermic	[166]
I	Fish	Rat	Liver	1 mg/mL	Hypothermic	[94]
I/III	Fish	Rat	Hearts	10, 15, and 20 mg/mL	Hypothermic	[69]
I/II/III	Fish	Human	Red blood cells	0–1.54 mg/mL	Cryopreservation	[197]
I	Fish	Human	Red blood cells	5–160 μg/mL	Cryopreservation	[68]
III	Eel pout	Mouse	Oocyte	0.1 mg/mL	Vitrification	[66]
FfIBP	Bacteria			0.05 mg/mL
LeIBP	Yeast			0.1 mg/mL
III	Eel pout	Bovine	Oocyte	0.5–1 μg/mL	Vitrification	[198]
AFGP8	Fish	Bovine	Oocyte	1 mM (2.6 mg/mL)	Vitrification	[199]
DcAFP	Beetle Dendroides canadensis	Buffalo	Semen	0.1, 1.0, and 10 μg/mL	Cryopreservation	[200]
LeIBP	Yeast	Human	Cell lines	0.1 mg/mL	Cryopreservation	[145]