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. 2017 Feb 10;15(2):36. doi: 10.3390/md15020036

Figure 5.

Figure 5

AEX inhibited IBDV replication in chicken bursa of Fabricius. SPF chickens (6 chickens per group) were oral inoculated with different concentrations of AEX (0, 62.5, 125, 250 and 500 mg/kg body weight) per day. After three days, chickens were eye-dropped with IBDV (105.5TCID50). Bursa of Fabricius were collected at 5 d.p.i. and divided into two parts. (A) Half of the bursa of Fabricius were grinded and virus titers were determined by TCID50; (B) The other half bursa of Fabricius were subjected to isolate total RNA and the expression lever of IBDV VP2 was evaluated by qRT-PCR. Data represent means ± SEM from five wells per group. * p ≤ 0.05; ** p ≤ 0.01. Results are representative of two independent experiments.