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. 2017 Feb 14;15(2):37. doi: 10.3390/md15020037

Figure 6.

Figure 6

Thin-layer chromatography (TLC) analyses for reaction products of recFlKin and recFlAld. (A) Reaction products produced by recFlKin. The reaction products were visualized by spraying 10% (v/v) sulfuric acid in ethanol followed by heating at 130 °C for 10 min. M, standard KDPG; (B) Reaction products of recFlAld. The reaction products were visualized with 0.5% (w/v) 2,4-dinitrophenylhydrazine (DNP)–20% (v/v) sulfuric acid. The color was graphically inverted to ease the recognition of spots. M1, standard pyruvate; M2, standard glyceraldehyde-3-phosphate (GAP). Stained materials near the original position are GAP oligomers.