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. 2017 Feb 15;15(2):39. doi: 10.3390/md15020039

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Induction of G₀/G₁ arrest and apoptosis by FX in A549 and H1299 cells. A549 (A) and H1299 (B) cells after the 48-h treatment of FX were stained with PI, then DNA content was analyzed by flow cytometry. G₀/G₁, S and G₂/M indicate different cell cycle phases. Annexin V-FITC/PI staining assay of A549 (C) and H1299 (D) cells was analyzed by flow cytometry to detect apoptosis after FX treatment for 48 h and Q4 represent cells in apoptotic stage. Data are presented as means ± SD (n = 3). * p < 0.05, ** p < 0.01 compared to the control group, ^# p < 0.05, compared to the FX 12.5 μM treatment group.