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. 2017 Mar 3;7:43669. doi: 10.1038/srep43669

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Copyright © 2017, The Author(s)

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(a) Hela-GFP and Hela cells were mixed 1:1 and treated with 5 μM SIM or 10 μM PRA up to 8 days, fixed in 3.7% paraformaldeide and incubated with the primary antibody anti-neuroserpin (Ab32901, Abcam) coniugated PLA probes (1:50), overnight at 4 °C. The presence of aggregates are visualized using Duolink in Situ staining according to the manufacturer’s instructions. Nuclei are stained with DAPI. Images are acquired by Leica SP2 laser scanning confocal microscope. Each red spot represents a neuroserpin aggregate. (b) The number of spots per cell for each condition reported in panel a is quantified as described in the methods section.