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. 2017 Mar 3;7:43669. doi: 10.1038/srep43669

Figure 4. Detection of cholesterol with TNM-AMCA in untreated and treated HeLa cells after statins treatments.

Figure 4

Untreated or 5 μM SIM or 10 μM PRA treated HeLa cells for the time reported in the figure, were fixed with 3.7% paraformaldeide and then incubated with TNM-AMCA (1 μM) to stain cholesterol for 1 h at room temperature. Images were acquired by Leica SP2 laser scanning confocal microscope.