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. 2017 Mar 3;7:43669. doi: 10.1038/srep43669

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Subconfluent cells are fixed with 3.7% paraformaldeide after 8 days treatment with statins and incubated with anti-SREBP1 (1:100, ab28481) or anti SREBP-2 (1 μg/ml ab30682, Abcam) overnight at 4 °C. Next, the cells are incubated with anti-rabbit Alexa Fluo488 for 1 h at room temperature. Actin is stained with 1 μg/ml Actistain-555 phalloidin for 1 h at room temperature. Nuclei are stained with DAPI. Untreated cells are also shown for reference. Images are acquired by Leica SP2 laser scanning confocal microscope.