Skip to main content
. 2017 Mar 3;7:43715. doi: 10.1038/srep43715

Figure 8. Transient expression of McMYB12 genes in apple fruit.

Figure 8

McMYB12s were overexpressed using the pBI121-McMYB12a and b vectors, or the gene was suppressed in apple fruit using the pTRV2-McMYB12s vector. Apple fruit injected with the empty pBI121 and TRV vectors and infiltration buffer only were used as controls. (A) Apple fruit peel coloration around the injection sites of overexpressing (pBI121-McMYB12a and b) or suppressed (pTRV2-McMYB12s) apples. (B) flavonoids compound levels around the injection sites of apple peels in μg/g flesh weight (FW). (C) Relative expression levels around the infiltration sites were determined using qRT-PCR, the expression level of MYB12 represent the transcription level of MdMYB12 and MdMYB121 in silenced fruits. MdMYB121 have 99% sequence identity to McMYB12a and MdMYB12 have 99% sequence identity to McMYB12b. Error bars indicate the standard error of the mean ± SE of three replicate measurements. Different letters above the bars indicate significantly different values (P < 0.05) calculated using one-way analysis of variance (ANOVA) followed by a Tukey’s multiple range test.