Table 1.
Oligonucleotide sequences for quantitative real-time PCR.
| Species | Gene | Primer Sequence (5’→3’) | Accession Number | Product Size | Location of Primer Start |
|---|---|---|---|---|---|
| Rat | Foxl2 | F: TCGCTAAGTTCCCGTTCTAC | XM_003750571.4 | 173 | 301 |
| R: GTAATTGCCCTTCTCGAACA | |||||
| Kitlg | F: TTCAAGGACTTCATGGTGGC | NM_021843.4 | 164 | 451 | |
| R: GCGGCTTTCCTATTACTGCT | |||||
| Amh | F: CTGGCTGAAGTGATATGGGA | NM_012902.1 | 198 | 391 | |
| R: CACAGTCAGCACCAAATAGC | |||||
| Star | F: GCGGAACATGAAAGGACTGA | NM_031558.3 | 184 | 51 | |
| R: TCCTTGCTGGATGTAGGACA | |||||
| Cyp11a1 | F: GCTTTGCCTTTGAGTCCATC | NM_017286.3 | 189 | 605 | |
| R: CATGGTCCTTCCAGGTCTTA | |||||
| Cyp19a1 | F: GGCAAGCACTCCTTATCAAACC | NM_017085.2 | 197 | 671 | |
| R: TCCACGTCTCTCAGCGAAAA | |||||
| Hsd3b1 | F: TGCCACTTGGTCACACTGTCA | NM_001007719.3 | 148 | 949 | |
| R: CCCTGTGCTGCTCCACTAGTGT | |||||
| Fshr | F: CTTGAAGCGGCAAATCTCTG | NM_199237.1 | 198 | 910 | |
| R: GAGCAGGTCACATCAACAAC | |||||
| Lhcgr | F: CTCACTGAAAACACTGCCCT | NM_001007719.3 | 198 | 817 | |
| R: ATGGCGGAATAAAGCGTCTC | |||||
| Rn18s | F: CTCAACACGGGAAACCTCAC | NR_046237.1 | 110 | 1251 | |
| R: CGCTCCACCAACTAAGAACG |
F (forward); R (reverse).