A proposed ecosystem of mycolactone producing mycobacteria in Adiopodoumé. Three water bodies (pond 1, pond 2 and Ebrié lagoon), frequently used by the community of Adiopodoumé for washing dishes, laundry, and gardening activities, were selected for the study. Pond 1 flowed into pond 2 and they both ran to Ebrié lagoon through the Adiopodoumé Km 17 Bridge. These environmental water bodies represent potential sources of infection by mycolactone producing mycobacteria (MPMs). The transmission of MPMs to humans could occur by direct contact with contaminated environmental samples through skin microtraumatism.