Table 1.
Specific primers used for identification of non-tuberculous mycobacteria.
| Primer Name | Forward and Reverse Primer Sequences | Size of Amplicon (bp) | References |
|---|---|---|---|
| 16S rRNA | PA: 5′-AGAGTTTGATCCTGGCTCAG-3′ MSHA: 5′-AAAAAGCGACAAACCTACGAG-3′ |
620 | [25] |
| IS2404 Nested 1 | pGp1: 5′-AGGGCAGCGCGGTGATACGG-3′ pGp2: 5′-CAGTGGATTGGTGCCGATCGAG-3′ |
400 | [5] |
| IS2404 Nested 2 | pGp3: 5′-GGCGCAGATCAACTTCGCGGT-3′ pGp4: 5′-CTGCGTGGTGCTTTACGCGC-3′ |
210 | [5] |
| ER | LMF: 5′-GAGATCGGTCCCGACGTCTAC-3′ LMR: 5′-GGCTTGACTCATGTCACGTAAG-3′ |
420 | [10] |
| Locus 6 | R-5′-GACATCGAAGAGGTGTGCCGTCT-3′ F-5′-GACCGTCATGTCGTTCGATCCTAGT-3′ |
variable | [10] |
| Locus 19 | R-5′-TGGCGACGATCGAGTCTC-3′ F-5′-CCGACGGATGAATCTGTAGGT-3′ |
variable | [10] |
| MIRU1 | R-5′-GCCCTCGGGAATGTGGTT-3′ F-5′-GCTGGTTCATGCGTGGAAG-3′ |
variable | [10] |
| ST1 | R-5′-CGCCACCCGCGGACACAGTCG-3′ F-5′-CTGAGGGGATTTCACGACCAG-3′ |
variable | [10] |
ER: enoyl reductase; MIRU1: mycobacterial interspersed repetitive unit 1; rRNA: ribosomal RNA; ST1: sequence type 1.