Table 2. Summary of sensitivities and specificities achieved for each technique.
| Raman Spectroscopy |
DHM Histogram |
DHM Texture analysis |
Combined RS and DHM |
|||||
|---|---|---|---|---|---|---|---|---|
| sens % | spec % | sens % | spec % | sens % | spec % | sens % | spec % | |
| CD4+ v B cell | 86.8 | 98.6 | 93.8 | 85.4 | 78.0 | 62.2 | 81.3 | 88.3 |
| CD4+ v monocytes | 97.9 | 98.1 | 98.7 | 100 | 98.5 | 97.0 | 100 | 100 |
| B cell v monocytes | 98.6 | 98.1 | 100 | 100 | 100 | 100 | 98.6 | 100 |
| Average | 94.4 | 98.3 | 97.5 | 95.1 | 92.2 | 86.4 | 93.3 | 96.1 |
RS and DHM are each capable of efficiently discriminating between cell subsets. Describing phase images in terms of a pixel intensity histogram is more efficient than using texture parameters.