Table 2. Correlations between Hb gene expression and MGIT mycobacterial growth.
| Description | UK/Asian adults (n = 21) Whole Blood MGIT |
South African infants (n = 128) PBMC MGIT |
||||
|---|---|---|---|---|---|---|
| logFC | p-value | adj. p-val | logFC | p-value | adj. p-val | |
| alpha haemoglobin stabilizing protein | 2.04 | *2.67E-02 | 1.05E-01 | 0.16 | 4.91E-01 | 6.88E-01 |
| cytochrome b5 reductase 3 | −0.34 | 2.52E-01 | 4.60E-01 | 0.01 | 8.54E-01 | 9.30E-01 |
| haemoglobin, alpha 1 and 2 | 0.15 | 6.50E-01 | 8.03E-01 | 1.53 | *2.22E-02 | 1.01E-01 |
| haemoglobin, beta | 0.08 | 7.36E-01 | 8.58E-01 | 1.47 | *2.19E-02 | *9.98E-02 |
| haemoglobin, delta | 2.54 | 1.13E-01 | 2.75E-01 | 0.73 | *3.54E-02 | 1.35E-01 |
| haemoglobin, epsilon 1 | −0.74 | 6.35E-01 | 7.93E-01 | ND | ND | ND |
| haemoglobin, gamma A | 1.81 | 1.06E-01 | 2.65E-01 | 1.87 | *7.10E-03 | *4.94E-02 |
| haemoglobin, gamma G | 1.78 | 1.15E-01 | 2.78E-01 | 1.78 | *7.66E-03 | *5.17E-02 |
| haemoglobin, mu | 1.87 | *3.93E-02 | 1.37E-01 | 0.36 | 1.95E-01 | 3.96E-01 |
| haemoglobin, theta 1 | 1.76 | *5.62E-02 | 1.75E-01 | 0.17 | 3.35E-01 | 5.48E-01 |
| haemoglobin, zeta | 2.08 | 4.29E-01 | 6.35E-01 | ND | ND | ND |
mycobacterial growth was treated as a continuous variable from two different studies of whole blood from 21 healthy adults from the UK and Asia in an M.tb H37Rv-stimulated MGIT PBMC and from 130 South African infants in a BCG-stimulated MGIT assay. Gene expression levels were taken from unstimulated samples for individual genes from the GO category ‘haemoglobin complex’. logFC refers to the amount by which gene expression increases per unit increase in mycobacterial growth. *Indicates significant correlations (p < 0.05). ND = not detected.