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. 2017 Mar 3;7:43252. doi: 10.1038/srep43252

Figure 3. Interaction of Mcp with As(III) and As(V) as measured by relative intrinsic fluorescence.

Figure 3

Purified Mcp (1.0 μM) was incubated with As(III) or As(V) (0, 5, 10, 15, 20 and 25 μM) for 1 h before testing. (A) Fluorescence quenching observed when Mcp was incubated with incremental increases of As(III). (B) Fluorescence quenching observed when Mcp was incubated with incremental increases of As(V). Fluorescence change at 330 nm was analyzed by GraphPad Prism 5, and KD value was calculated to be 11.01 ± 3.02 μM.