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. 2017 Mar 3;7:43252. doi: 10.1038/srep43252

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Copyright © 2017, The Author(s)

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(A) Bacterial one-hybrid assay. Co-transformants containing pBX-Mt2031p/pTRG-Rv3133c were employed as positive controls (CK+), while co-transformants containing pBXcmT/pTRG-AioR was used as negative controls (CK−). Cells of CK+, pBX-Pmcp/pTRG-AioR, and CK- were grown to OD₆₀₀ of 1.0 and 2 μL of each was spotted onto His-selective medium (+3AT, +Str^r) and LB plate (−3AT, −Str^r). (B–E) EMSA assays. FAM-labeled aioBA probe (B) and mcp probe (C) both interacted with AioR protein. (D) Competition experiments including labeled and unlabeled mcp probe. (E) Mutant FAM-labeled mcp probe (the mutation sites are underlined) interaction with AioR protein. The amounts of DNA probes and AioR were shown in the above table of each panel.