FIG 2.

The ArfGAP domain of ADAP2 is needed for the antiviral response. (A) Ectopic expression of ADAP2 enhanced RIG-I overexpression-mediated IFN-β luciferase reporter activity in HEK293T cells. (B) Schematic showing the domain organization of the ADAP2 protein. (C) Effects of ectopic expression of different truncations of ADAP2 on RIG-I overexpression-mediated IFN-β luciferase reporter activity. The inset shows expression levels of truncation proteins determined by Western blotting (IB). Reporter (firefly) luciferase values were normalized with a renilla luciferase internal control and expressed as fold induction values. The values shown are means and SD for a representative experiment performed in triplicate. The statistical significance of differences in mean values was analyzed using unpaired two-tailed Student's t test, and P values of <0.05 were considered statistically significant (*, P < 0.05; **, P < 0.01). EV, empty vector; WT, wild type; Mut, truncation mutant; PH, pleckstrin homology domain; none, only the IFN-β promoter-driven luciferase reporter was present within the cells; GAPDH, glyceraldehyde-3-phosphate dehydrogenase (cytoplasmic internal control marker).