FIG 5.

ADAP2 interacts with specific domains of RIG-I pathway proteins. Cells were transfected with plasmids for full-length HA-ADAP2 and various truncations of NEMO, TBK1, IRF3, and MAVS, and coimmunoprecipitation was performed using an antibody detecting the HA tag. (A) The N-terminal 150 amino acids of NEMO were needed to interact with ADAP2. (B) Amino acids between positions 300 and 385 of TBK1 were needed for its association with ADAP2. (C) The region of IRF3 between amino acids 325 and 390 was needed for strong interaction with ADAP2. (D) MAVS interacted with ADAP2 through its N-terminal 100 amino acids. FL, full length; SeV, Sendai virus; WCL, whole-cell lysate; IP, immunoprecipitation; IB, immunoblot; HA, hemagglutinin tag; GAPDH, glyceraldehyde-3-phosphate dehydrogenase (cytoplasmic internal control marker).