FIG 8.

Subcellular distribution of ADAP2 during infection. (A) Subcellular distribution of ADAP2. Immunoblot of subcellular fractions for the presence of the indicated proteins are shown; (B) MAVS silencing abolished the mitochondrial localization of ADAP2 during SeV infection. Subcellular fractionation identifies a distribution of ADAP2 in cytosolic, plasma membrane and mitochondrial fractions of Sendai virus infected cells; (C) Sendai virus infection upregulated expression of ADAP2 protein in HEK293Tcells. si, siRNA; NT-si, nontargeting negative-control siRNA; h, hours; SeV, Sendai virus; Mt, mitochondrial fraction; M, membrane fraction; C, cytosolic fraction; AIF, apoptosis-inducing factor, a mitochondrial marker; tubulin, cytosolic marker; N-cadherin, plasma membrane marker.