FIG 6.

Magnesium decreases acetylation in a dose-dependent manner. Wild-type E. coli cells (strain BW25113) were aerated in TB7 alone or supplemented with 0.4% glucose and/or the indicated concentrations of MgSO₄ for 10 h. (A) Coomassie blue-stained SDS-polyacrylamide gel and loading control for panel B. (B) Antiacetyllysine Western blot of lysates from cells harvested from the indicated conditions at 10 h. The high-molecular-mass band observed in the absence of glucose but significantly diminished in the presence of glucose is acetyl-CoA synthetase, whose gene is repressed by glucose and whose acetylation does not depend upon acetyl phosphate.