pLR16-pheS* annotated plasmid map. The plasmid layout of pLR16-pheS*, including the key functions annotated, is shown. Ori+ is a temperature-sensitive Gram-positive origin of replication first published as a part of pKSV7, and the origin of transfer (OriT), chloramphenicol resistance genes, and multiple cloning site (MCS) were published as a part of pPL2 (18, 35). The figure was generated using SnapGene software (GSL Biotech).