TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Escherichia coli strains | ||
| XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| SM-10 | Conjugation donor; F− thi-1 thr-1 leuB6 recA tonA21 lacY1 supE44 (Muc+) λ− [RP4-2(Tc::Mu)] Kmr Tra+ | 33 |
| Listeria monocytogenes strains | ||
| 10403S | WT, Strepr | D. A. Portnoy, lab stock |
| A118 cured | 10403S ΔcomK phage | 23 |
| Δmonocin mutant | 10403S ΔcomK phage ΔLMRG_2362-2337 | This study |
| ΔftbQR mutant | 10403S ΔcomK phage ΔLMRG_2377-2378 | This study |
| WT + pPL2 pConst pheS* | 10403S harboring pPL2 integrative plasmid expressing a mutated B. subtilis pheS, p-Cl-pheS, Cmr | This study |
| WT + pPL2 pConst GFP | 10403S harboring pPL2 integrative plasmid expressing GFP | This study |
| Plasmids | ||
| pPL2 | E. coli/L. monocytogenes conjugative vector, integrative to tRNA-Arg gene, Cmr | 18 |
| pBHE261 | E. coli/L. monocytogenes conjugative vector, pKSV7 temp-sensitive Ori, Amp−r, Cm+r | 35 |
| pLR16-pheS* | E. coli/L. monocytogenes conjugative vector, pKSV7 temp-sensitive Ori, p-Cl-pheS, Cmr | This study |
| pLR16Δmonocin | pLR16 with upstream and downstream homology fragments inserted | This study |
| pLR16ΔftbQ-R | pLR16 with upstream and downstream homology fragments inserted | This study |
aAmpr, ampicillin resistance; Kmr, kanamycin resistance; Cmr, chloramphenicol resistance; p-Cl-Phes, p-chloro-phenylalanine sensitivity. −, Gram-negative resistance; +, Gram-positive resistance.