TABLE 2.

Primers used in this study

Primer	Sequence	Purpose
1020-NdeI	TTCCATGGCCAACACTTGTCACTACTTTCACTTATGGTGTTCAATGCTTTTCAAGATACCCAGATCACATGAAG	Eliminate NdeI site in plasmid pJAM-1020
1020-StuI	CTGAACAGGCCTAGGATAGCCC	Reverse primer
1020-NdeI-BglII	AACGGATCCTAGAAATAATTTTGTTAACTTTAAGAAGGAGATATACACAAGGAGATATAACATATGGGCAGATCTATGAGTAAAGGAGAAGAACTTTTC	Introduce NdeI and BglII sites into plasmid pJAM-1020 to generate plasmid pJAM-1020I
TatSP-NdeI	GGACATATGCGACGGAATCACAGCC	Introduce NdeI site into TatSP sequence
TatSP-BglII	CTCAGATCTGGCGGCCGCACTGGTCG	Introduce BglII site into TatSP sequence