Neighbor-joining phylogenetic tree of Tunisian L. micranthus-associated (Lmi) isolates and reference strains based on recA (375 bp) sequences. Bootstrap values were calculated for 1,000 replications, and those greater than 70% are indicated at the internodes. Black diamonds (◆) indicate Tunisian Lmi isolates. Collapsed groups include group A: B. elkanii USDA76T, B. ferriligni CCBAU 51502T, B. pachyrhizi PAC48T, B. viridifuturi SEMIA 690T, B. embrapense CNPSo 2833T, and B. tropiciagri CNPSo 1112T; and group B: B. ingae BR 10250T, B. iriomotense EK05T, B. kavangense 14-3T, B. manausense BR 3351T, B. subterraneum 58 2-1T, B. yuanmingense CCBAU 10071T, B. vignae 7-2T, B. daqingense CCBAU 15774T, and B. huanghuaihaiense CCBAU 23303T. Group VII includes only two strains (LmiM8 and LmiE10) that contain 4 copies of recA (see the text for details). B., Bradyrhizobium; M., Microvirga; P., Phyllobacterium. Accession numbers from GenBank and whole-genome sequencing (NCBI) databases are shown in parentheses.