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. 2017 Mar 3;7:43880. doi: 10.1038/srep43880

Figure 5. Titration of an FAD or FMN solution with apo-EE to determine the dissociation constants of the apoenzyme-FAD/FMN complex.

Figure 5

Flavin fluorescence emission was monitored at 525 nm, excitation was at 445 nm. (A) 200 nM FAD titrated with 5 μM of apo EE and (B) 200 nM FMN titrated with 5 μM apo EE.