Figure 6. Fluorescence emission spectra of of holo- and apo-EE.

Excitation was at 445 nm. (A) Spectra of 0.9 μM FAD, 0.9 μM FMN, 0.9 μM holo-EE and 0.9 μM holo-EE denatured in 0.5% SDS. (B) Spectra of 0.9 μM apo-EE and 0.9 μM apo-EE reconstituted with FAD or FMN. For comparison, the spectrum of holo-EE is depicted.