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. 2017 Mar 3;7:43880. doi: 10.1038/srep43880

Figure 7. Preparation of ΔABC for crystallisation.

Figure 7

(A) Removal of the MBP-tag and purification of native ΔABC, visualised on SDS-PAGE. From left to right: (1) purified MBP-TtProDH ΔABC; (2) MBP-TtProDH ΔABC after limited trypsinolysis; (3) purified native ΔABC. Molecular masses of marker proteins (M) from top to bottom: 150, 100, 75, 50, 37, 25, 20 kDa. (B) Growth of TtProDH ΔABC crystals.