Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
| Specific activity (U mg−1) | kcat (s−1) | KM (mM) | kcat/KM (s−1 M−1) | |
|---|---|---|---|---|
| Holo-EE* | 4.74 ± 0.27 | 9.8 ± 0.5 | 67.6 ± 8.2 | 146 |
| Apo-EE | 0.84 ± 0.01 | ND | ND | ND |
| Apo-EE + FAD | 4.83 ± 0.07 | 7.2 ± 0.2 | 36.6 ± 3.2 | 198 |
| Apo-EE + FMN | 4.95 ± 0.10 | 7.9 ± 0.2 | 50.1 ± 3.4 | 158 |
Activity measurements were done at 25 °C, pH 7.4 with the proline: DCPIP oxidoreductase assay.
*As determined previously47.
