Fig. 5.
The high-affinity BLT2 ligand 12-HHT agonist does not influence macrophage (MΦ) bacterial phagocytosis. BMDMs were isolated from WT, 5-LOX−/−, or BLT1−/− mice. The BLT2-specific agonist, 12-HHT (0.1 μM, 1 μM), was added to B. burgdorferi (Bb)-stimulated 5-LOX−/− (A) or BLT1−/− (B) macrophages with or without LTB4 (1 nM) and their phagocytic index determined after 1 or 4 h coculture. C: Activation of cAMP by treatments. Bars represent mean + SEM and are the results from two independent experiments (n = 3 per group). *P < 0.01 versus corresponding WT control cells.