Skip to main content
. 2017 Feb 27;58(3):504–511. doi: 10.1194/jlr.M071266

Fig. 4.

Fig. 4.

Hepatic CYP7A1 protein expression and serum C4 levels in LS-Xbp1−/− and Xbp1fl/fl mice. A: Serum C4 levels were measured to determine CYP7A1 synthetic activity in LS-Xbp1−/− and Xbp1fl/fl mice (n = 3–4). Representative Western blot (B) and densitometry quantification (C) of CYP7A1 protein expression in liver homogenates from LS-Xbp1−/− and Xbp1fl/fl mice (n = 4). GAPDH was used as a loading control. Representative Western blot (D) and densitometry quantification (E) of CYP7A1 protein expression in microsomes from LS-Xbp1−/− and Xbp1fl/fl mice (n = 3). GRP78 was used to confirm microsome enrichment and as a loading control. Although CYP7A1 protein expression in whole liver homogenates and microsomes was similar in both genotypes, CYP7A1 synthetic activity was reduced in LS-Xbp1−/− mice. *P < 0.05 compared with Xbp1fl/fl mice.