Fig. 6.

Serum C4 levels and hepatic CYP7A1 protein expression in LS-Xbp1^−/− and Xbp1^fl/fl mice fed chow, cholestyramine, or cholesterol. LS-Xbp1^−/− and Xbp1^fl/fl mice were fed chow, chow with cholestyramine (2% w/w), or chow with cholesterol (2% w/w) for 7 days. Western blot (A) and densitometry quantification (B) of hepatic CYP7A1 protein expression in chow-fed and cholestyramine-fed mice. GAPDH was used as a loading control. C: Changes in serum C4 levels in LS-Xbp1^−/− and Xbp1^fl/fl mice fed chow, cholestyramine, and cholesterol. Data are expressed as percent increase compared with chow-fed Xbp1^fl/fl mice. Western blot (D) and densitometry quantification (E) of hepatic CYP7A1 protein expression in chow-fed mice and cholesterol-fed mice. GAPDH was used as a loading control. F: Hepatic gene expression of Abcg5 and Abcg8 was measured. *P < 0.05, **P < 0.01, ***P < 0.001 compared with chow-fed Xbp1^fl/fl mice. ⁺P < 0.05, ⁺⁺P < 0.01 compared with chow-fed LS-Xbp1^−/− mice. ^#P < 0.05 compared with cholestyramine-fed Xbp1^fl/fl mice. ^¶¶P < 0.01 compared with cholesterol-fed Xbp1^fl/fl mice. n = 3–5.