Fig. 8.

Fa2h transcripts in adult rat testis (T) and differentiating spermatogenic cells. PtSs, RSs, and LSs were isolated from the seminiferous epithelium of adult rats. A: Data were obtained by RT-qPCR and normalized to Hprt as internal reference gene using the 2^−ΔΔCt method, as described in the text. The relative expression of Fa2h in adult rat testis (set to equal 1) was used as a calibrator for comparison among the cell populations. The asterisks point to a significantly different value (P < 0.01). B: Analysis of RT-PCR products on a 2% agarose gel. After reverse transcription using random primers, the corresponding cDNAs were amplified using specific primers for rat Fa2h. In all samples, the products were in agreement with the predicted size of 96 bp. MW, molecular weight markers.