Fig. 2.

PS synthesis at ER-mitochondria contacts increases PS transport to mitochondria. A: The indicated strains were labeled with [³H]serine for 30 min and the percentage of [³H]PS converted to [³H]PE was determined (mean ± SD, n  =  3 independent experiments). *P < 0.05, independent two-tailed t-test. Raw data used to calculate percent of [³H]PS converted to [³H]PE is in supplemental Fig. S2A, B. B, C: Steady state levels of phospholipids in whole cells (B) and mitochondria (C) from the indicated strains grown in SG with choline (mean ± SD, n  =  3 independent experiments). *P < 0.05, independent two-tailed t-test. PI, phosphatidylinositol; PA, phosphatidic acid; CL, cardiolipin.