Fig. 5.

In the presence of PA as the anionic lipid, cholesterol (Chol), and Cer regulatory effects on membrane bound GlcCer hydrolysis by GBA1 are insignificant, while SM plays a strong inhibitory role. Liposomes contain 5 mol% cholesterol, 1 mol% [¹⁴C]GlcCer, 20 mol% PA, and 0–30 mol% cholesterol, Cer, or SM (as indicated) with DOPC being the host lipid. The experiments were carried out using 60 ng GBA1 per assay under 30 min incubation and at 37°C. Mean ± SEM (n = 4–6).