Figure 6. NF-κB target gene PIM1 is up-regulated by patterned action potential activity.

Protein expression levels of PIM1 following 90/5 stimulation for 5 hr were measured by immunoblotting. 10 μM MG132, a general proteasome and NF-κB inhibitor, was pre-incubated for 2 hr prior to stimulation. MG132 treatment reduced basal levels of PIM1 expression. GAPDH was used as a loading control.