Table 3. Enriched canonical pathways by 18/1 patterned stimulation for 2 hr and 5 hr.
| Pathway | # mol | Z-score | p | Molecules |
|---|---|---|---|---|
| 2 hr | ||||
| IGF-1 Signalling | 7 | −1.13 | 4.41e-3 | FOS, CSNK2A2, SOCS3, SHC1, PRKAG2, STAT3, SOCS5 |
| ERK/MAPK Signalling | 8 | −2.12 | 3.76e-2 | FOS, SHC1, ITGA3, PRKAG2, DUSP4, PAK7, RPS6KA1, STAT3 |
| Tec Kinase Signalling | 7 | −1.63 | 4.62e-2 | FOS, ITGA3, GNB2, STAT2, PAK7, STAT3, ITK |
| Axonal Guidance Signalling | 15 | — | 3.80e-2 | RGS3, SLIT1, BDNF, NFATC3, C9orf3, EPHA1, WNT9A, PLCH2, LIMK1, SHC1, ITGA3, PRKAG2, GNB2, PAK7, VASP |
| 5 hr | ||||
| CDK5 Signalling | 20 | −0.47 | 1.07e-2 | ITGB1, PPP1R14C, RAF1, BDNF, PPP2R5D, PPP2R5B, ADCY6, MAPK12, CACNA1A, CDK5R1, ADCY9, PPP1R3D, ITGA3, PPP1R12A, MAP2K2, CAPN1, PRKAG2, MRAS, PPP2R5E, MAPK7 |
| Death Receptor Signalling | 19 | 2.07 | 9.45e-3 | MAP2K4, GAS2, RELA, MAP2K7, PARP8, TNKS2, FAS, ACIN1, DAXX, TRADD, TRAF2, CASP9, CRADD, TNFSF12, BID, HTRA2, CHUK, NFKBIB, TNFRSF10A |
| Rac signalling | 20 | 1.34 | 2.29e-2 | MAP2K4, ITGB1, RELA, RPS6KB1, RAF1, MAP2K7, MAP3K11, ARPC5L, MAP3K1, PIKFYVE, PIP4K2B, WASF1, CDK5R1, PAK1, ITGA3, MAP2K2, BAIAP2, MRAS, PIP5KL1, PAK7 |
| ERK5 signalling | 13 | 1.73 | 3.76e-2 | IL6ST, RPS6KB1, BAD, CREB3, CREBBP, MYC, RPS6KA6, MEF2D, NTRK1, MRAS, FOSL1, MAPK7, RPS6KA1 |
| NGF signalling | 19 | 1.41 | 4.20e.2 | MAP2K4, GAS2, RELA, MAP2K7, PARP8, TNKS2, FAS, ACIN1, DAXX, TRADD, TRAF2, CASP9, CRADD, TNFSF12, BID, HTRA2, CHUK, NFKBIB, TNFRSF10A |
IPA analysis was performed on up- and down-regulated transcripts (>|1.4|-fold and p < 0.05) as described in Fig. 2 and mapped to cellular and molecular signalling pathways. Z-score represents the predicted pathway activation (+) or inhibition. Significance values were calculated by a Fisher’s exact test (right-tailed) to determine the probability that a pathway was attributable to chance alone. Enriched pathways are shown, demonstrating opposing activation (18/1) and inhibition (90/5) for Nerve Growth Factor (NGF) and Rac signalling (Fig. 3) important for neurite outgrowth.