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. 2017 Mar 3;7:43787. doi: 10.1038/srep43787

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(a) Total and phosphorylation levels of MEK1/2, ERK1/2, JNK1/2, and P38 were evaluated by western blots (n = 7–8 samples). The β-actin was served as loading control. (b) The gray value ratio of target phosphorylation protein to β-actin; (c) The gray value ratio of target phosphorylation protein to its total protein. n.s. indicated no significance.