Figure 5. miR-125b-5p and Bcl2 expression are biomarkers for prognosis in human gallbladder cancer tissues.

(A,B) Bcl2 mRNA (A) expression and protein expression (B) in clinical gallbladder cancer tissues and neighboring normal tissues was determined by qPCR and immunohistochemistry. Bcl2 expression was up-regulated in gallbladder cancer tissues than neighboring normal tissues. (C) Correlation of miR-125b-5p expression and Bcl2 expression in human gallbladder cancer tissues was analyzed using Pearson’s correlation analysis. r = −0.594, P < 0.001. The expression of miR-125b-5p and Bcl2 were inversely correlated. (D,E) Gallbladder cancer patient survival was analyzed by Kaplan-Meier analysis. P value was calculated using log-rank test. Bcl2 expression which exceeds the average level (mean value) is defined as “high” while Bcl2 expression which is less than the average level (mean value) is defined as “low”. (D) Higher Bcl2 expression in tumors is correlated with poor prognosis. (E) Patients with both lower miR-125b-5p expression and higher Bcl2 expression in tumors exhibit poor prognosis.