Figure 4. Evaluation of 1α,25(OH)₂D₃ and MART-10 effect on p21 and p27 expressions in SNU308 cells.

After two days of treatment (10⁻⁶ to 10⁻⁸ 1α,25(OH)₂D₃ or 10⁻⁷ to 10⁻⁹ M MART-10), p21 and p27 expressions were determined by western blot. (a) A western blot showing p21 and p27 expression in SNU308 cells with or without treatment. Actin was used as the loading control. (cropped). (b) Quantitative result of western blot. Both 1α,25(OH)₂D₃ and MART-10 increased p27 expression in SNU308 cells without obvious effect on p21. MART-10 was more effective than 1α,25(OH)₂D₃. Each value is a mean ± SD of three to five determinations. *p < 0.05, **p < 0.01 versus control.