Skip to main content
. 2017 Mar 3;7:43773. doi: 10.1038/srep43773

Figure 6. Evaluation of 1α,25(OH)2D3 and MART-10 effect on NGAL expression in SNU308 cells and proliferation on SNU308 and SNU308- NGALsi cells, and NGAL effect on ki-67 expression of SNU308 cells.

Figure 6

NGAL expression in SNU308 cells was evaluated by western blot after two days of 10−6 to 10−8 1α,25(OH)2D3 or 10−7 to 10−9 M MART-10 treatment. (a) A western blot depicting NGAL expression in SNU308 cells with or without treatment. Actin was used as the loading control. (cropped). (b) Quantitative result of western blot. Both 1α,25(OH)2D3 and MART-10 repressed NGAL expression in SNU308 cells with MART-10 more effective than 1α,25(OH)2D3. To investigate NGAL role in SNU308 cells, Ki-67 expression was measured by flow cytometry in SNU308 cells and SNU308 NGAL-si cells. (c) The histogram of Ki-67 expression in SNU308 cells and SNU308 NGAL-si cells. (d) The quantitative result of Ki-67 expression in SNU308 cells and SNU308 NGAL-si cells. Our result indicates that knockdown of NGAL in SNU308 cells decreased Ki-67 expression. (e) SNU308NGAL-si cells were treated by 1α,25(OH)2D3 or MART-10 as indicated concentrations every two days. 7 days later, the cell proliferation was measured by WST-1 method. Both 1α,25(OH)2D3 and MART-10 inhibited SNU308 NGAL-si cells proliferation dose-dependently with MART-10 much more potent than1α,25(OH)2D3. However, the inhibition effect was less than that observed in SNU308 cells. (f) SU308 and SNU308NGAL-si cells were treated by either vehicle, or rhNGAL (0.5 ng/ml), or rhNGAL and MART-10. The cell proliferation was measured two days later. rhNGAL increased cell growth of both SU308 and SNU308NGAL-si cells. The rhNGAL-increased cell growth was abolished by MART-10. (Each value is a mean ± SD of three to five determinations. *p < 0.05, **p < 0.01 versus control).