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. 2017 Mar 3;7:43092. doi: 10.1038/srep43092

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Copyright © 2017, The Author(s)

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Prussian blue staining on paraffin sections of embryos injected with (a) MNP, (b) MNP-rVEGF, (c) MNP-PLL and (d) MNP-rVEGF:mAb. Left: control side; right: injected side. Nanoparticles (blue stained) are pointed by arrowheads in the RPE, black arrows in the choroid and white arrows in the retina. (e) Percentage of embryos showing preferential particle localisation in retina, RPE or choroid. n = 45. Chi-square test. (f) TEM imaging of larvae injected with MNP-rVEGF. POM: periocular mesenchyme. (f’) Magnification of inset in F showing particle clusters.