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. 2017 Mar 3;7:43153. doi: 10.1038/srep43153

Figure 4. Effects of autophagy induction on IL-1β, IL-6, TNF-α, and NO production in alpha-synuclein-stimulated BV2 cells.

Figure 4

BV2 cells incubated with 3-MA (2 mM) for 1 h at 37 °C were cultured in the presence or absence of rapamycin (100 nM) or trehalose (30 mM) for 24 h. After that, microglial cells were stimulated with alpha-synuclein fibers (20 uM) for 24 h and the supernatants were isolated and analyzed by ELISA, for the measurement of IL1-β (A), IL-6 (B) and TNFα (D), respectively. For IL-1β determination, ATP (5 mM) was added for the last 3 h of alpha-synuclein stimulation. NO levels (C) were determined by Griess assay. Results were analyzed by one-way ANOVA followed by Post-Hoc Dunnet’s test; n = 3. Error bars represent SEM (***P < 0.001). F20 = alpha-synuclein fibers (20 uM), R = rapamycin, T = trehalose.