Figure 2. Lack of tubular epithelial cell recovery associated with reduced number of collecting ducts following acute IRI using mice genetically deficient in the expression of TRIP13.

Histology of Trip13^+/+ (A,C,E and G) and Trip13^Gt/Gt(B,D,F and H) contralateral and ischemia‐reperfusion injured mouse kidneys are shown after 72 (A–D) and 168 hours (E–H) following IRI. α-pifithrin (αPFT; 2.2 mg/kg IP) was administered daily for 7 days in Trip13^+/+ (G) and Trip13^Gt/Gt (H) mice. (I) Graphical representation of the tubular injury as a percent of the total tubules is shown. The number of animals per group was shown in the bars. *P < 0.05, Ctx = cortex; OM = outer medulla; CK = contralateral kidney, *indicates cast‐filled tubules; scale bar = 60 μm (A–H). (J) Plasma creatinine levels in bilateral renal IRI-treated mice. Blood was collected at 24 and 168 hours after IRI. n = 3 mice per time point and group. *P < 0.05; ***P < 0.005 significant difference from WT versus Trip13^Gt/Gt at the same time point.