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. 2017 Mar 3;7:43196. doi: 10.1038/srep43196

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Immunostaining of γH2AX was performed in Trip13^+/+ (A and C) and Trip13^Gt/Gt(B and D) contralateral and IRI-treated mouse kidneys are shown after 168 hours. Arrowheads in C and D indicate γH2AX-positive cells. (E) Graphical representation of the γH2AX-positive cells as a percent of the total nuclei is calculated. n = 4 mice per group. **P < 0.01, scale bar = 60 μm (A–D).