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. 2017 Mar 3;7:43248. doi: 10.1038/srep43248

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Copyright © 2017, The Author(s)

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(A) After 24 h of primary culture, the cells developed two distinct shapes that represented two types of cells: hE-SCs(white arrows) and hE-FLCs(black arrows). (B) After two rounds of purification, the hE-SCs purity was improved. (C) The remnant hE-FLCs continued to rapidly proliferate and were passaged in vitro. (D) The purity of hE-SCs is shown as the mean ± S.D. from three independent experiments, *P < 0.05, significantly different compared to the hE-SCs/P2 group and the other two groups. (Scale bars = 100 μm).