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. 2017 Mar 3;7:43185. doi: 10.1038/srep43185

Figure 2. Transplantability of testicular cells retrieved from cryopreserved whole testes.

Figure 2

(A–F) Intraperitoneal transplantation of donor testicular cells (olvas-GFP) into a recipient hatchling under a fluorescence microscope. (G,H) Donor-derived TCs (arrow) showing green fluorescence were incorporated into recipient gonads at 15 dpf (G) and incorporated TCs began to proliferate at 20 dpf (H). (I) Survival rates of recipients at each developmental stage after transplantation. (J,K) The incorporation rate of GFP-positive cells in recipient gonads for each developmental stage of the recipient (J) and those for each cell number (K). Data are shown as the mean ± SEM (Different letters indicate significant differences, P < 0.01). (L1-3, L3 is magnified photograph of L2) Gonads of WT female triploid. (M,N) Proliferation of donor germ cells in a triploid recipient ovary (M1-4, M4 is magnified photograph of M3) and testes (N1-2) at two months. Scale bars, 1 mm (C), 100 μm (B, G, H, L1, M1, M3-4, N1), and 10 μm (L2, L3, M2, N2).