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. 2017 Mar 3;7:43173. doi: 10.1038/srep43173

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Rats allografted with C6 cells were injected with PBS (control) or NA as described in Methods. (A–G) Brain slices were collected and H&E staining was carried out. Representative images of brain slices collected from rats injected with PBS or NA are shown in (A–F) (with indicated magnification), and quantitative results from both groups (6 rats/group) are summarized in G. Arrows indicate C6 glioma cells. ***P < 0.001. (H–M) Brain slices were processed for DAPI staining (blue) and immunocytochemistry for Nestin (red). (N) NA significantly improved the survival of C6-allografted rats as compared with control (PBS; n = 25 for each group, P < 0.001).